Assessment of one maize hybrid lot uniformity by UPOV morphological and protein markers

Monitoring genetic uniformity and identity is very important in maize breeding seed production. Due to practical reasons, molecular biochemical methods for the determination of purity should reliably reflect differences associated with traditional morphological traits, even when they are not agriculturally important. A comparison one hybrid lot, based on UPOV markers UTLIEF (Ultrathin-layer isoelectric focusing) method performed this research. Tested samples expressed uniform protein profiles, but other hand, unsatisfactory field, indicating some laches Although method, standardized by ISTA rules, provides enough accurate distinction between F1 self-pollinated maternal seed, a "clean" isozyme or profile will necessarily correlate homogeneity. It most likely that non-uniformity tested lot originates from parental lines. Therefore, establish where occurred, it necessary perform post-control tests reference lines, as well as, insight into their official descriptions according descriptor. Thus, require deposition referent hybrids varieties whose production allowed Serbia, have harmonised protocols conduction laboratory field tests.